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pdcas9dnmt3a egfp  (Addgene inc)


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    Structured Review

    Addgene inc pdcas9dnmt3a egfp
    Pdcas9dnmt3a Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdcas9dnmt3a+egfp/pdCas9-DNMT3A-EGFP+(Plasmid+%2371666)/pm41620608-256-17-18
    Average 93 stars, based on 33 article reviews
    pdcas9dnmt3a egfp - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Comprehensive profiling of CRISPR/dCas9 epigenome editors indicates a complex link between on and off target effects.
    Article Snippet: The same procedure was applied to the control tool, consisting of dCas9 fused with the inactive DNMT3A (M3-dCAS9DNMT3A, Addgene #220237). .. M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A3A, Addgene #218776) plasmid made by the amplification of the human DNMT3A catalytic domain from the pdCas9DNMT3A-EGFP (Addgene #71666) plasmid using the primers containing the FseI restriction sites. .. For M3-dCAS9-DNMT3A-DNMT3L (dCas9-3A3L, Addgene #218777), the murine DNMT3L catalytic domain and 27bp linker included FseI restriction site were amplified from pET28-Dnmt3a3L-sc27 AR TIC LE IN PR ES S plasmid (Addgene #71827).

    Amplification:

    Article Title: Comprehensive profiling of CRISPR/dCas9 epigenome editors indicates a complex link between on and off target effects.
    Article Snippet: The same procedure was applied to the control tool, consisting of dCas9 fused with the inactive DNMT3A (M3-dCAS9DNMT3A, Addgene #220237). .. M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A3A, Addgene #218776) plasmid made by the amplification of the human DNMT3A catalytic domain from the pdCas9DNMT3A-EGFP (Addgene #71666) plasmid using the primers containing the FseI restriction sites. .. For M3-dCAS9-DNMT3A-DNMT3L (dCas9-3A3L, Addgene #218777), the murine DNMT3L catalytic domain and 27bp linker included FseI restriction site were amplified from pET28-Dnmt3a3L-sc27 AR TIC LE IN PR ES S plasmid (Addgene #71827).

    Article Title: Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells.
    Article Snippet: For generation of plasmids with scFv-linked EDs encoding the catalytic domain (CD) of DNMT3A, DNMT3A3L, KRAB and LSD1, the TET1 plasmid was digested for 1 h at 37 ◦C with FD-NotI and with FD-NheI (both Thermo Fisher Scientific). .. The sequences of DNMT3A, DNMT3A3L, KRAB and LSD1 were PCR amplified from pdCas9DNMT3A-EGFP (Addgene #71666), pET28-Dnmt3a3L-sc27 (Addgene #71827), pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911) and pX330a dCas9-LSD1 (Addgene #92362), respectively. .. The digested vector backbone and the PCR product were joined using the Ligation-Free Cloning Kit (Applied Biological Materials Inc.) according to the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells.
    Article Snippet: For generation of plasmids with scFv-linked EDs encoding the catalytic domain (CD) of DNMT3A, DNMT3A3L, KRAB and LSD1, the TET1 plasmid was digested for 1 h at 37 ◦C with FD-NotI and with FD-NheI (both Thermo Fisher Scientific). .. The sequences of DNMT3A, DNMT3A3L, KRAB and LSD1 were PCR amplified from pdCas9DNMT3A-EGFP (Addgene #71666), pET28-Dnmt3a3L-sc27 (Addgene #71827), pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911) and pX330a dCas9-LSD1 (Addgene #92362), respectively. .. The digested vector backbone and the PCR product were joined using the Ligation-Free Cloning Kit (Applied Biological Materials Inc.) according to the manufacturer’s protocol.



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    93
    Addgene inc pdcas9dnmt3a egfp
    Pdcas9dnmt3a Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdcas9dnmt3a+egfp/pdCas9-DNMT3A-EGFP+(Plasmid+%2371666)/pm41620608-256-17-18
    Average 93 stars, based on 1 article reviews
    pdcas9dnmt3a egfp - by Bioz Stars, 2026-10
    93/100 stars
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